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Mouse inoculation for the detection of non-cultivable gastric tightly spiralled bacteria BJMBR
Mendes,E.N.; Queiroz,D.M.M.; Moura,S.B.; Rocha,G.A..
In the present study we compared the inoculation of swine gastric mucus into the stomach of mice, the urease test and carbolfuchsin-stained smears for the diagnosis of the infection with "Gastrospirillum suis" ("Helicobacter heilmannii" type 1), an uncultivated tightly spiralled gastric bacterium. Fragments obtained from the antral and oxyntic mucosa of the stomach of 50 slaughtered pigs were used for urease test, for carbolfuchsin-stained smears and for obtaining scrapings of mucus for mouse inoculation. The mice were killed by spinal dislocation 10 days after inoculation and fragments of the antral and oxyntic mucosa were used for spiral bacterium identification (urease test and carbolfuchsin-stained smears). Among the methods employed for the diagnosis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helicobacter; Helicobacter heilmannii; Gastrospirillum/infection diagnosis; Gastric spiral bacteria; Helicobacter infection diagnosis; Mouse inoculation.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300007
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The role of IFN-gamma and IL-4 in gastric mucosa inflammation associated with Helicobacter heilmannii type 1 infection BJMBR
Cinque,S.M.S.; Rocha,G.A.; Correa-Oliveira,R.; Soares,T.F.; Moura,S.B.; Rocha,A.M.C.; Nogueira,A.M.M.F.; Cabral,M.M.D.A.; Vieira,L.Q.; Martins-Filho,O.A.; Queiroz,D.M.M..
Although Helicobacter heilmannii infection is less common than H. pylori infection in humans, it is considered to be of medical importance because of its association with gastritis, gastric ulcer, carcinoma, and mucosa-associated lymphoid tissue lymphoma of the stomach. However, there have been no studies evaluating the role of the Th cell response in H. heilmannii gastric infection. We evaluated the participation of pro-inflammatory and anti-inflammatory cytokines, IFN-gamma and IL-4, in H. heilmannii gastric infection in genetically IFN-gamma- or IL-4-deficient mice. The serum IFN-gamma and IL-4 concentrations were determined by ELISA. The gastric polymorphonuclear infiltrate was higher (P = 0.007) in H. heilmannii-positive than in H. heilmannii-negative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helicobacter heilmannii; Gastric mucosa inflammation; IFN-gamma-/- mice; IL-4-/- mice.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200012
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